【摘要】 目的:探讨丹皮酚(Paeonol,Pae)抑制人肝癌细胞株Bel-7404增殖作用及其分子机制。方法:应用光学显微镜、MTT法检测经不同浓度、不同时间Pae处理Bel-7404细胞后的增殖抑制作用;DNA凝胶电泳法、TUNEL法检测细胞凋亡;用RT-PCR法、免疫细胞化学ABC法检测抑癌基因PTEN和致癌基因Akt表达水平。结果:与空白对照组比,Pae能抑制人肝癌细胞Bel-7404的增殖,诱导其凋亡,光镜下可见明显的凋亡细胞;DNA凝胶电泳显示出典型的凋亡特征;RT-PCR和免疫细胞化学检测显示,Pae处理Bel-7404后可使PTEN表达升高,Akt表达下降。结论:Pae具有抗Bel-7404细胞增殖的作用,其机制可能是通过抑制PI3K通路,增强诱导其凋亡有关。更多还原

【Abstract】 Objective To investigate the anti-tumor effect of Paeonol (Pae) on the hepatocellular carcinoma cell line Bel-7404 and its molecular mechanisms. Methods Hepatocellular carcinoma cell line Bel-7404 was treated by Pae in various concentrations and different time points respectively; and then the cell proliferation was assayed by light microscope, MTT method. DNA agarose gel electrophoresis and TUNEL were used to detect the apoptosis. The expression of PTEN and Akt were examined by RT-PCR and immunocytochemical ABC method. Results Compared with the control groups Pae obviously increased the inhibitory and apoptosis rate of hepatocellular carcinoma cell line Bel-7404. It also showed a typical apoptotic morphology and DNA depicted a ladder pattern characteristic of the apoptosis, indicating the presence of DNA fragmentation. RT-PCR and immunocytochemical ABC assay showed that Pae could increase the expression of PTEN and decrease the expression of Akt. Conclusion Pae can increase the anti-hepatocellular carcinoma effect, and its mechanism may be the increase of apoptosis-inducing effect which is regulated by phosphatidylinositol-3-kinase.更多还原