【摘要】 目的:观察重组人粒细胞集落刺激因子(recombinant human granulocyte colony stimulating factor;rhG-CSF)对D-氨基半乳糖(D-galactosamine,D-GalN)加脂多糖(lipopolysaccharide,LPS)联合诱导的小鼠急性肝衰竭的保护作用,并探讨其机制。方法:D-GalN/LPS腹腔联合注射制造小鼠急性肝衰竭模型,治疗组小鼠于造模前4h,2h及造模同时予以G-CSF300μg/kg腹腔注射,观察小鼠24h存活率。造模后6h每组随机选择5只小鼠处死,观察肝组织损伤情况,用半定量逆转录-聚合酶链反应和TanonGis3.73软件分析各组小鼠肝组织中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白介素-6(IL-6)和白介素-10(IL-10)的表达情况。结果:治疗组小鼠24h存活率明显高于对照组(P<0.01),造模后6h治疗组小鼠肝组织损伤明显减轻(P<0.05),肝组织中TNF-α和IFN-γ的mRNA表达水平明显低于对照组(P<0.01),IL-6和IL-10的mRNA表达水平明显高于对照组(P<0.01)。结论:G-CSF对D-GalN/LPS所致的小鼠急性肝衰竭具有保护作用。更多还原

【Abstract】 Objective To evaluate the protective effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on acute hepatic failure induced by galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice, and to explore its mechanism. Methods The mice were intraperitoneally administered D-GalN (800mg/kg) and LPS (10μg/kg), and then were intraperitoneally injected either saline (the control group )or rhG-CSF at 300μg/kg body weight (the therapy group ) at 4h, 2h and 0h before the D-GalN/ LPS injection. The survival rate of the mice was estimated at 24h after the D-GalN/ LPS injection. The degree of hepatic injury was evaluated at 6h after the D-GalN/ LPS injection, and the levels of TNF-α, IFN-γ, IL-6 and IL-10 mRNA were simultaneously measured by semiquantitative RT-PCR. Results The survival rate of the therapy group was significantly higher than that of the control group (68.4% vs 20%,P<0.01). As compared with the control group, the degree of liver injury in the therapy group significantly decreased (P<0.05), and the levels of TNF-α and IFN-γ mRNA in the hepatic tissue also reduced remarkably (P<0.01, respectively), while the levels of IL-6 and IL-10 mRNA increased (P<0.01, respectively) at 6h after the D-GalN/ LPS injection. Conclusion G-CSF can protect the mice from acute hepatic failure induced by D-GalN/LPS.更多还原