【摘要】 目的:探讨氨基胍(AG)对全肝血流阻断再灌注大鼠肺损伤的影响。方法:阻断肝门及肝上、肝下下腔静脉20 m in建立大鼠全肝血流阻断再灌注模型。将90只大鼠随机均分成假手术组(A组)、全肝血流阻断组(B组)和全肝血流阻断加氨基胍治疗组(C组),每组再根据观察时间段的不同随机均分成3个亚组。A,B两组按1mL/kg从股静脉注射生理盐水,C组注射AG 20 mg/kg(溶于1 mL/kg生理盐水中)。观察各组全肝血流阻断20 m in(T0)、再灌注4 h(T1)门静脉血一氧化氮(NO)、内毒素(ET)、肿瘤坏死因子(TNF-α)浓度,大鼠48 h生存率,大鼠肺组织湿干质量比及电镜下组织结构变化。结果:与A组比较,B,C两组T0,T1门静脉血NO,ET,TNF-α及肺湿干质量比明显升高(P<0.05或P<0.01),但C组明显低于B组(P<0.05);B,C两组大鼠48 h存活率降低,但C组明显高于B组(P<0.05);B,C两组肺组织均存在病理改变,但C组明显轻于B组。结论:氨基胍具有保护全肝血流阻断再灌注大鼠肺组织的作用。更多还原

【Abstract】 Objective To investigate the effects of aminoguanidine on the lung injury induced by the total hepatic ischemia-reperfusion in rats.Methods The total hepatic ischemia-reperfusion model was built after blocking of the hepatic porta,suprahepatic and infrahepatic vena cava.Ninety Sprague-Dawley rats were assigned randomly into 3 groups: Sham operation group（Group A,n=30）;total hepatic ischemia group（Group B,n=30）;and aminoguanidine treatment group（Group C,n=30）.Each group was subdivided randomly into 3 subgroups（n=10） according to different time phases: 20 minutes after the total hepatic vascular exclusion(T₀),4hours after the reperfusion(T₁),and 48 hours after the survival.Group A and Group B were intravenously injected with normal saline（1mL/kg） while Group C was injected with aminoguanidine（20mg/kg） dissolved in normal saline（1mL/kg） 10 minutes before the open of the abdomin.The levels of portal blood nitric oxide（NO）,endotoxin（ET）,tumor necrosis factor-α（TNF-α） at T₀ and T₁ were detected;48 hours survival rates and the lung wet/dry weight ratio were counted;and the histological changes of the lung tissues were observed.Results Compared with Group A,the levels of portal vein NO,ET,and TNF-α at T₀ and T₁ in Group B and Group C were significantly higher（P<0.05 or P<0.01）.But those indexes of Group C were lower than those of Group B（P<（0.05）.） The 48-hour survival rate in Group C was higher than that in Group B（（P<）（0.05）.） The lung wet/dry weight ratio in Group C was lower than in Group B（P<（0.05）） and the histological change of Group C was slighter than that in Group B.Conclusion Aminoguanidine has the protective effects on the lungs against the total hepatic ischemia-reperfusion induced injury.更多还原