【摘要】 为获得稳定的转基因刺槐植株,以速生型刺槐叶轴为转化受体,对转化过程中的一些影响因素进行了探究,建立了较为完善的遗传转化体系。结果表明:继代20~35d之间刺槐叶轴最适宜转化;卡那霉素的筛选浓度为30mg/L;头孢霉素的抑菌浓度为200mg/L;合适的侵染时间是20min;30mg/L的乙酰丁香酮有利于抗性芽的发生;共培养2d对转化适宜;抑菌7d后再加选择压有利于抗性芽的产生。最后得到的抗性植株经PCR检测,初步证明外源目的基因OsDREB1已整合到刺槐基因组中。更多还原

【Abstract】 The rachis of fast-growing Black Locust was used as explants in the study.The factors that affected transformation ratio were studied,and a stable transformation system was established.The results suggested that germinated shoots which 20～35dsdd in vitro were suitable for transgenic.The optimal concentration of kanamycim to the rachis was 30mg/L and that of cefotaxine was 200mg/L.The ideal time for rachis dipping in Agrobacterium was 20min and optimal co-cultivation was 2 days.30mg/L acetosyringone could increase the transformation ratio.The selection of the inoculated rachis was done at 7^th days of bacterial inhibiting treatment by cefotaxine.The PCR analysis showed that the target gene OsDREB1 was integrated into the genome of black locust.更多还原