【摘要】 在含不同浓度秋水仙素的MS+0.1mg/L NAA+15mg/L BA(最适器官发生)双层培养基上进行兰考泡桐四倍体植株诱导试验,并通过变异植株根尖细胞染色体观察和叶片单细胞DNA含量测定进行倍性分析。结果表明,预培养12d的兰考泡桐叶片在秋水仙素浓度为10mg/L的双层培养基上预培养处理24h时,四倍体诱导率最高达到23.4%。变异植株叶片比二倍体大,叶片长宽比变小,叶片单个气孔变大,气孔密度变小。更多还原

【Abstract】 Autotetraploids from diploidy leaves of Paulownia elongata on the double layer MS media supplemented with 0.1 mg/L NAA and 15 mg/L BA(optimal organogenetic medium to the leaves of diploidy seedlings) with different colchicines concentrations were induced,chromosome numbering of the plant root tip cells and DNA content analysis of the leaf single cell were used to analyse the autotetra ploids. The results indicated that the highest tetraploid induction rate could reach 23.4% when the leaves precultured on the optimal organogenetic MS medium for 12d and treated with 10 mg/L colchione on the double layer media for 24h in vitro.Compared with diploidy seedlings of P.elongate,leaf length and stomata size of tetraploid becomes bigger,the leaf length/width ratio and stomata density becomes smaller.更多还原