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不同能量重离子注入农作物的诱变效应

EFFECTS OF HEAVY ION IMPLANTATION ON CROP MUTAGENESIS

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【作者】 刘志芳邵俊明唐掌雄施巾帼李文建颉红梅卫增泉李桂英

【Author】 LIU Zhi-fang~1 SHAO Jun-ming~1 TANG Zhang-xiong~(1,2) SHI Jin-guo~2LI Wen-jian~3 XIE Hong-mei~3 WEI Zeng-quan~3 LI Gui-ying~1(1.Institute of Crop Science,Chinese Academy of Agricultural Sciences/Key Lab of Agricultural Nuclear Technology & Aerospace Breeding,Ministry of Agriculture,Beijing 100081;2.Institute for Application of Atomic Energy,Chinese Academy of Agricultural Sciences,Beijing 100094;3.Institute of Moden Physics,Chinese Academy of Sciences,Lanzhou,Gansu 730000)

【机构】 中国农业科学院作物科学研究所/农业部农业核技术和航天育种重点开放实验室中国农业科学院原子能利用研究所中国科学院近代物理研究所中国农业科学院作物科学研究所/农业部农业核技术和航天育种重点开放实验室 北京100081北京100081北京100081 中国农业科学院原子能利用研究所北京100094甘肃兰州730000

【摘要】 用不同能量、不同注量的MEV级12C离子束辐照玉米和冬小麦干种子,用MEV级16O离子束辐照冬小麦干种子,研究其对M1代幼苗生长的影响和M2代的诱变效应。结果表明,在一定离子通量(注量)下,所用注入能量范围内的离子束对幼苗造成的辐射损伤随辐射能量的增加而增大。用12~16MEV/U的12C离子束辐照玉米和8MEV/U16O离子束辐照小麦,对M1代幼苗造成的辐射损伤比贯穿能量(45MEV/U)下的辐射损伤明显加重。12C离子束可以诱发玉米产生植株矮化、雄性不育、白化苗、多穗型等多种类型变异,多数白化苗能够转绿并正常结实。12C和16O离子束诱发冬小麦产生的早熟和矮杆突变最多,12C辐照冬小麦原冬6产生的早熟突变在辐照能量为8MEV/U、离子通量为80×107/CM2时高达10.7%;矮秆突变在辐照能量为8MEV/U、离子通量为120×107/CM2时高达7.59%。品种间的变异频率也存在差异。

【Abstract】 (()12C) ions with different energy(in MeV level) and flux were implanted into dry seeds of corn and winter wheat.(()16O) ions with different energy were implanted into dry seeds of winter wheat.The radiation damage of M1 generation seedling and mutagenic effects in M2 generation were studied.Results showed that within a certain energy level,radiation damage on M1 seedling increased with the energy increasing when the integral flux was given.And radiation damage for(()12C) ion beam with an energy of 12~16MeV/u in corn,and(()16O) ion beam with an energy of 8MeV/u in wheat were obviously greater than that of ion beam with penetration energy(45 MeV/u).The corn mutations induced by(()12C) ion beam could generate various types,such as dwarf,male sterility,albino,multi-ear,and most albinotic seedlings could turn to normal plants and set seeds.Earliness and dwarf were the most frequent mutations found in winter wheat induced by the two type of ions,and in winter wheat Yuandong 6,the earliness mutation frequency was up to 10.7% induced by(()12C) ion beam with an energy at 8MeV/u and integral flux of 80×107/cm2,and dwarf mutation frequency reached 7.59% induced by an energy at 8MeV/u and integral flux of 120×107/cm2.Variations of mutation frequencies were found among varieties of corn and wheat.

【基金】 国家自然科学基金项目(10275090)
  • 【文献出处】 核农学报 ,Journal of Nuclear Agricultural Sciences , 编辑部邮箱 ,2006年01期
  • 【分类号】S335
  • 【被引频次】44
  • 【下载频次】347
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