【摘要】 为探讨纤维连接蛋白(Fn)、精-甘-天冬氨酸肽(RGD)对人支气管上皮细胞(BEC)损伤修复的影响,用永生化人BEC进行培养,将培养细胞分为7组:1.对照组;2.臭氧(O3)应激组;3.O3+EGF组;4.O3+Fn组;5.O3+RGD组;6.O3+Fn+钙调素抑制剂W7组;7.O3+Fn+蛋白酪氨酸激酶抑制剂Genestin组,分别测定各组的损伤修复指数(RI),利用计算机图像分析软件对数据进行处理和比较.结果发现O3应激组的损伤修复指数显著低于对照组(P<0.01),O3+EGF组、O3+Fn组和O3+RGD组损伤修复指数则显著高于O3应激组(P<0.01),Fn的促损伤修复效应可被W7和Genestin阻断(P<0.01).该实验表明EGF,Fn和RGD对BEC的损伤后修复有保护作用,Fn的作用机制可能与酪氨酸磷酸化途径和钙调素途径有关.更多还原

【Abstract】 The study was designed to study the effect of fibronection（Fn） and Arg-Gly-Asp peptide（RGD） on wound repair of bronchial epithelial cells.Cultured bronchial epithelial cells were divided into seven groups,including control group,ozone（O₃） exposure group,O₃ and EGF group,O₃ and Fn group,O₃ and RGD,O₃ and Fn and W₇ group,O₃ and Fn and Genestin group.The repair index（RI） of every group was measured by using the software of figure analysis.Compared the O₃ exposure group with the control group,the RI of the O₃ exposure group descended significantly（P<0.01）.Compared with O₃ exposure,EGF,Fn and RGD increased significantly the RI（P<0.01）.The effect of Fn could be inhibited by calmodulin-inhibitor W₇ and protein-tyrosine kinase inhibitor genestin（P<0.01）.The results indicate that EGF and Fn and RGD promote wound repair of bronchial epithelial cells wirth O₃ exposure.The mechanism of Fn may be related with tyrosine phosphorylation or calmodulin pathway.更多还原