【摘要】 用鸡传染性法氏囊病病毒(IBDV)及其重组表达蛋白VP2,VP3和单抗做各种ELISA试验,测定单抗识别的IBDV蛋白抗原及其多肽片段,并分析单抗识别IBDV抗原表位的空间关系。结果表明,抗IBDV单抗A,B,C,D,E,G与重组表达蛋白VP2呈阳性反应,而与表达蛋白VP3呈阴性反应,单抗C与表达蛋白VP2和VP3均呈阳性反应;单抗A,B,D,E,G识别IBDV VP2蛋白上相近或相似的抗原表位,均识别VP2蛋白多肽序列PJ1,PJ2,PJ5,PJ14,属于同一类群的构像性单克隆抗体。更多还原

【Abstract】 The epitopes recognized by the 5 anti-IBDV monoclonal antibodies（Mabs） of A,B,D,E and G were defined by ELISAs using Mabs,IBDV and its proteins expressed in Escherichia coli and synthetic peptides.All of the 5 Mabs reacted with recombinant VP₂ protein but not with recombinant VP₃ protein by indirect ELISA,and were shown to recognize the same or closely adjacent epitopes on IBDV VP₂ protein in ELISA additive test.The reactivity of each of the 5 Mabs with IBDV was clearly inhibited by the peptides of PJ1,PJ2,PJ5 and PJ14 respectively in antigen captured-ELISA.The results indicated that the 5 Mabs belong to one group of conformational-dependent Mabs which recognize the same or closely adjacent epitopes on VP₂ protein of IBDV,and all recognize the peptides of PJ1,PJ2,PJ5 and PJ14.更多还原