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大蒜茎尖脱毒及组织培养研究
Study on Virus-free Technology of Shoot-tip and Tissue Culture in Allium sativum
【摘要】 采用茎尖分生组织培养技术,获得了大蒜无病毒试管苗。通过激素配比试验,筛选出最佳的培养基组成,进行脱病毒苗的快速繁殖。结果表明:诱导茎尖出芽的最适培养基为MS+6 BA 1.5mg/L+NAA 1.0mg/L,诱导生根的最适培养基为MS+IAA 2.0mg/L.
【Abstract】 Virus-free materials of Allium sativum L.were obtained from in vitro shoot-tip tissue culture.A series of optimization experiments for concentration of hormones were investigated.The results showed that the induction frequency of stem-tips was the best when the medium was MS containing 6BA 1.5mg/L and NAA 1.0mg/L and the ratio of the induction of roots was the most when the medium was MS supplemented with IAA 2.0mg/L.
【基金】 国家农业科技成果转化资金项目(04EFN214100192)
- 【文献出处】 河南农业科学 ,Journal of Henan Agricultural Sciences , 编辑部邮箱 ,2006年11期
- 【分类号】S633.4
- 【被引频次】13
- 【下载频次】630