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磺胺间甲氧嘧啶全抗原的制备研究
Study on Preparation of Complete Antigen of Sulfamonomethoxine
【摘要】 采用重氮化法,将半抗原磺胺间甲氧嘧啶(SMM)与载体牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联,制得磺胺间甲氧嘧啶全抗原SMM-BSA和SMM-OVA。通过其紫外扫描光谱、SDS-PAGE凝胶电泳试验,表明半抗原和载体成功偶联,免疫动物以后可以产生满意效价。这为进一步制备抗SMM抗体提供了良好的免疫原。
【Abstract】 Semiantigen sulfamonomethoxine(SMM) was coupled with carrier protein bovine serum albumin(BSA) and ovalbumin to prepare complete antigen SMM-BSA and SMM-OVA by the method of diazotization.UV scanning spectrum,SDS-PAGE and animal immunity all indicated that the complete antigens both SMM-BSA and SMM-OVA were successfully produced.The present research supplied an excellent immune antigen for further preparation of antibody to SMM.
【关键词】 磺胺间甲氧嘧啶;
全抗原;
紫外扫描;
免疫;
鉴定;
【Key words】 Sulfamethoxydiazine; Complete antigen; UV scanning spectrum; Immunity; Identify;
【Key words】 Sulfamethoxydiazine; Complete antigen; UV scanning spectrum; Immunity; Identify;
【基金】 “十五”国家重大科技专项“食品安全关键技术综合示范”(2001BA804A30-08)
- 【文献出处】 河南农业科学 ,Journal of Henan Agricultural Sciences , 编辑部邮箱 ,2006年03期
- 【分类号】S859.84
- 【被引频次】5
- 【下载频次】174