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RAPD技术分析亲本鸭及其杂交后代的基因组变化

RAPD Analysis of the Genomic of Parent Ducks and Their Hybrids

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【作者】 苏瑛刘楚吾叶昌辉刘丽张锦蔡笛扬

【Author】 SU Ying~1,LIU Chu-wu~1,YE Chang-hui~1,LIU Li~1,ZHANG Jin~2,CAI Di-yang~2 (1.Guangdong Ocean University,Zhanjiang 524088,China;2.Zhanjiang Potou Bureau of Animal Husbandry,Zhanjiang 524045,China)

【机构】 广东海洋大学广东省湛江市坡头区畜牧局广东省湛江市坡头区畜牧局 广东湛江524088广东湛江524088广东湛江524045

【摘要】 对樱桃谷鸭和麻鸭及其杂交子代(樱♂×麻♀)3个样本的基因组DNA进行了RAPD标记分析,探讨了杂种优势产生的分子遗传机制。从120个随机引物中筛选出20个引物进行扩增,460条扩增带中,有330条带具有多态性。群体内相似性指数分别为0.7888(樱桃谷鸭)、0.7629(麻鸭)和0.8227(F1)。F1与母本的相对遗传距离为0.3300,与父本为0.3505,表明F1偏向母本。

【Abstract】 The genomic changes of parent ducks and their hybrid were analyzed with RAPD method.20 from 120 random primers were used to amplify genomic DNA of Cherry Valley Meat duck,Partridge ducks and their hybrids(C♂ × P♀).460bands were produced and 330 among them were polymorphism(71.73%).The inter-population similarity indexes were 0.7888(C),0.7629(P)and(0.8227)(F1))respectively,which showed greater variation among the parent and their hybrids.And the genetic distance between F1 and its mother was 0.3300,while it was 0.3505 with its father,which indicated that the genome was more similar to its mother.

【关键词】 樱桃谷鸭麻鸭杂交RAPD基因组
【Key words】 Cherry Valley Meat duckPartridge ducksHybridRAPDGenome
【基金】 广东省科技攻关项日(A200099A01);湛江市科技攻关项目(2000-17)
  • 【文献出处】 河南农业科学 ,Journal of Henan Agricultural Sciences , 编辑部邮箱 ,2006年01期
  • 【分类号】S834
  • 【被引频次】4
  • 【下载频次】100
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