【摘要】 为建立主要柑橘病毒病准确、快速的分子检测体系,在表现柑橘衰退病(CTV)、柑橘裂皮病(CEV)和柑橘碎叶病(CTLV)典型症状的病树上采叶样提取总RNA进行反转录聚合酶链式反应(RT-PCR)分析.病毒鉴定用的特异引物为CTV3对、CEV2对和CTLV1对.通过不同退火温度的测试,发现所有参试引物均能在56℃退火时扩增出相应的病毒特异带,从而在保证鉴定准确性的前提下,极大地简化了鉴定程序.用建立的RT-PCR体系对云南、重庆、湖南等地有衰退病、裂皮病和碎叶病症状的样品进行检测,结果表明该体系均能对相应病毒进行准确检测.更多还原

【Abstract】 In order to establish a quick and reliable identification system for the main Citrus viruses,total RNA was isolated from the sampled trees showing typical symptoms of Citrus tristeza virus(CTV),Citrus exocortis viroid(CEV) and Citrus tatter leaf virus(CTLV) for RT-PCR analysis.Three pairs of primers specific for CTVt,wo for CEV and one for CTLV,were tested for the corresponding viruses’ identification.Results indicated that all of the tested primers could generate specific products at the unique anneal temperatures of 56 ℃.More samples showing CTV,CEV or CTLV symptoms from Yunnan province,Chongqing city and Hunan province were tested by the established indexing technique,and the results indicated that the RT-PCR method was reliable for the identification of these Citrus viruses.更多还原