【摘要】 采用RT-PCR方法从超级稻幼苗中扩增DAD1基因的cDNA,并克隆测序,核酸序列表明该基因编码区为342bp,编码114个氨基酸残基.与GenBank发表的序列（XM₄72334）相比,有3处碱基不同,分别为105bp处的C（GenBank的为T）,148bp处的A（G）,149bp处的C（T）.将该基因分别正向和反向插入CaMV35S启动子后,构建了基因在植物中的正义、反义表达载体.更多还原

【Abstract】 DAD1 cDNA from super hybrid rice（Oryza sativa L.） was amplified by RT-PCR,and the coding sequence of the cDNA was analyzed.The result demonstrated that the coding region of DAD1 cDNA is 342 bp,encodes a protein consisting of 114 amino acids.Compared with the sequence in GenBank,there are three different bases from the gene XM₄72334.In order to study the function of this gene,binary vectors were constructed,in which the coding region of the gene was placed under the 35S promoter in either sense or antisense orientation.更多还原