【摘要】 以猪伪狂犬病毒为模式病毒,研究了CpG ODN对仔猪外周血中CD4+,CD8+T淋巴细胞的影响.实验结果表明:疫苗组初生仔猪血液中CD4+/CD8+T淋巴细胞比例随年龄的增加逐渐降低,而CpG ODN能有效抑制其降低(P<0.05);CpG ODN的使用也可以阻止伪狂犬病毒导致的血液中CD4+T淋巴细胞比例的降低;联合免疫CpG ODN和猪伪狂犬病毒活疫苗,可以诱导初生仔猪血清中猪伪狂犬病毒特异性抗体迅速产生并达到较高的水平(P<0.05);这表明CpG ODN能显著增强动物的免疫应答能力.更多还原

【Abstract】 The effect of immunostimulatory CpG oligodeoxynucleotides(CpG ODN) on CD4+ and CD8+ T lymphocyte subpopulations in the blood of newborn piglets was investigated with or without pseudorabies attenuated(PSA) vaccine.Experimental results suggest that the CD4+/CD8+ ratio decreases with the age of newborn pigs inoculated with the vaccine only,but keeps steady in pigs treated with CpG ODN and PSA vaccine(P>0.05),that CpG ODN can effectively prevent the decrease in the proportion of CD4+ T cell caused by PSA vaccine,and that high titer of PSA specific antibody can be tested in the serum of newborn piglets immunized by both PSA vaccine and CpG ODN(P<0.05).All these indicate that CpG ODN can greatly enhance the immune response of animals.更多还原