【摘要】 采用气相色谱-质谱联用技术并结合Kovats保留指数(IK)对比法分析鉴定了乌杨梅(Myrica rubraVar.astropureaTsen.)鲜叶芳香精油的成分,同时运用1,1-二苯基-2-苦基苯肼(DPPH)清除法、β-胡萝卜素漂白法和硫代巴比妥酸法对其抗氧化活性进行了对比分析.分析结果表明:乌杨梅叶精油成分以倍半萜类化合物为主(占总相对含量的97.45%),其中β-石竹烯(17.67%)、(E)-橙花叔醇(16.68%)、5-羟基去氢白菖烯(7.13%)、β-蛇麻烯(6.63%)、异石竹烯(6.46%)和α-杜松烯(5.81%)是其特征组分;乌杨梅叶精油具有一定的自由基清除能力和优异的脂质抗氧化活性,抗脂质氧化能力相当于强抗氧化剂2,6-二叔丁基对甲酚(BHT)的60%~85%.乌杨梅叶精油的强抗氧化活性可能与其含有较多的5-羟基去氢白菖烯有关.更多还原

【Abstract】 The chemical compositions of the essential oil from Myrica rubra Var.astropurea Tsen.leaves were analyzed and identified by the GC-MS analysis and the Kovats retention index.The antioxidant activity of the essential oil was also investigated with three different methods: the 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenger,the β-carotene bleaching test and the thiobarbituric acid reactive species assay.The results show that the essential oil is constituted mainly by sesquiterpenes(97.45%) and dominated with β-caryophyllene(17.67%),(E)-nerolidol(16.68%),5-hydroxycalamenene(7.13%),β-selinene(6.63%),isocaryophyllene(6.46%) and α-cadinene(5.81%),respectively,and that the essential oil possesses definite scavenging efficacy on radicals and significant antioxidant activity in lipid systems(account for 60%~85% of that of butylated hydroxytoluene(BHT)), which may result from the large content of 5-hydroxycalamenene.更多还原