【摘要】 选用巨峰、里扎马特和无核白葡萄的幼胚接种在附加了GA3、IAA、6-BA等的不同激素水平的MS培养基上进行培养,结果表明花后60d的巨峰和花后50d的无核白的幼胚在附加GA30.5mg.L-1、IAA2.0mg.L-1、6-BA0.5mg.L-1的培养基上分别得到了44.0±8.9%和96.0±8.9%的发育率与31.9±6.4%和37.5%的成苗率;花后60d的里扎马特幼胚在附加GA30.5mg.L-1、IAA0.5mg.L-1、6-BA0.5mg.L-1的培养基上得到了76.0±8.9%的发育率和63.5±5.5%的成苗率.筛选出了适合不同品种的最佳胚培养、萌发以及成苗培养基,为葡萄新品种的选育提供了新的方法与技术依据.更多还原

【Abstract】 Young embryos of Thompson Seedless(v.vinifera cv.Sultanina) grapes of the 50 days after florescence,and Kyoho(V.vinifera×V.labrusce cv.),Rizamat(V.vinifera cv.) grapes of the 60 days after florescence were cultured on MS medium supplemented with different concentrations of GA3,IAA and 6-BA in order to study the techniques of embryo culture.The results indicated that the best media of inoculation of embryos of Thompson Seedless,Kyoho and Rizamat grapes were selected.We got 44±8.9%,96.0±8.9% development rate and 31.9±6.4%,37.5% germinating rate(p<0.01) of Thompson Seedless’ and Kyoho’s embryo in the medium with MS+GA3 0.5mg·L-1 + IAA 2.0mg·L-1 + 6-BA 0.5mg·L-1,76.0±8.9% development rate and 63.5 ± 5.4% germinating rate(p < 0.01) of Rizamat’s embryos in the medium with MS + GA3 0.5mg·L-1+ IAA 0.5mg·L-1 + 6-BA 0.5mg·L-1.更多还原