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二乔刺槐组织培养及其体外植株再生技术研究
Tissue Culture and in Vitro Plantlet Regeneration of Robinia Pseudoacacia
【摘要】 以二乔刺槐叶片为试验材料,进行组织培养及其体外植株再生技术研究.结果表明,二乔刺槐幼嫩叶片进行组织培养的适宜基本培养基为WPM,芽诱导最适培养基为WPM+IBA0.25mg.L-1+BA5mg.L-1+GA30.05mg.L-1,根诱导最适培养基为WPM+IBA0.3mg.L-1.该结果为二乔刺槐的细胞工程和基因工程操作及其新品种的快速繁殖奠定了基础.
【Abstract】 A study was conducted on the technology of tissue culture and in vitro plantlet regeneration of Robinia pseudoacacia leaves as material.The results showed that media for the shoot induction from leaves and the rooting from shootings were WPM+IBA 0.25mg·L-1+BA 5mg·L-1+GA3 0.05mg·L-1 and WPM+IBA 0.3mg·L-1 respectively.These results might provide references to the cell engineering and gene engineering studies on Robinia pseudoacacia quick propagation of its new species.
【关键词】 二乔刺槐;
组织培养;
培养基;
植物生长调节剂;
【Key words】 Robinia pseudoacacia; tissue culture; medium; plant growth regulator;
【Key words】 Robinia pseudoacacia; tissue culture; medium; plant growth regulator;
【基金】 河南省高校杰出科研人才创新基金项目(2002KYCX003)
- 【文献出处】 河南科学 ,Henan Science , 编辑部邮箱 ,2006年02期
- 【分类号】S792.27
- 【被引频次】12
- 【下载频次】122