【摘要】 以菊花嫩茎段为材料,进行无菌系建立、芽分化、生根等试验,探讨菊花组培快繁技术.结果表明:(1)以MS+BA2.0 mg.L-1+NAA0.1 mg.L-1(单位下同)为培养基,分别置于普通培养室(不开灯,200~500 lux)与人工气候箱中培养,在前者条件下基本上不诱导芽的形成,25天后有部分黄白色愈伤组织形成;而在人工气候箱中培养则产生丛生芽,每茎段在30 d后可形成8~10个丛生芽.(2)分别以①MS+BA1.0+NAA0.1,②MS+BA2.0+NAA0.1,③MS+BA3.0+NAA0.1为增殖培养基,培养25 d后芽增殖3.65~5.90倍.(3)在a.1/2MS+NAA0.2;b.1/2MS+IAA0.2;c.1/2MS+IBA0.2共三个培养基中进行生根培养,生根率均达100%,但前两个培养基中长出的根系细长、数量少,而后一个培养基则根系粗短、数量多.更多还原

【Abstract】 Taking tender stems of Chrysanthemum for experiment samples,experiments such as non-bacteria system formation,buds differentiation,rooting were conducted.The results showed as follows: The first,sprouts couldn’t be induced and some callus could be formated after 25 d,s culturing on the medium of MS+BA2.0+NAA0.1 in common culture room;but sprouts could be induced and 8~10 sprouts were produced per tender stem after 30 d,s culturing in artificial climate room.The second,taking ①MS+BA1.0+NAA0.1,②MS+BA2.0+NAA0.1,③MS+BA3.0+NAA0.1 for propagation mediums,sprouts were propagated 3.65 to 5.90 times after 25 d,s culturing. The third,the rooting rate of tube-seedlings amounted to 100 % on the mediums of a.1/2MS+NAA0.2,b.1/2MS+IAA0.2,c.1/2MS+IBA0.2.but roots little and long for the former two,strong and numberal for the latter one.更多还原