【摘要】 目的探讨喉癌组织中血管内皮生长因子(VEGF)表达与CD83阳性树突状细胞(DC)分布的关系。方法42例喉癌组织经抗VEGF、抗CD83抗体免疫组化染色,观察喉组织中CD83阳性DC及VEGF分布,以及CD83阳性DC密度与VEGF表达强度的相关性。结果42例肿瘤标本中“-”12例,“+”者14例,“++”者16例。CD83+树突状细胞形态不规则,表面有许多不规则树状突起,主要分布于癌周组织内,趋向于成群分布;偶可见到CD83+DC位于靠近癌周组织的癌巢间间质内。喉癌组织中VEGF的表达明显升高的病例,其CD83+DC密度显著降低。结论VEGF的表达与喉癌组织中CD83+DC含量呈明显的负相关。更多还原

【Abstract】 Objective To investigate the relationship between the expression of vascular endothelial growth factor(VEGF) and CD83~+ dendritic cell density in human laryngeal carcinoma.Methods 42 laryngeal carcinoma tissues were investigated by immunohistochemical studies with anti-VEGF,anti-CD83 antibodies.Results For intensity of VEGF staining,12 tumors were graded as negative staining,14 as "+",16 as "++";CD83~+ mature DCs were irregular in shape and extended many irregular dendritic protrusions;CD83~+ mature DCs were confined to peritumoral areas in groups.Sometimes,CD83~+ DCs were found in stroma among cancer nests nextto peritumoral areas.A close correlation between VEGF and CD83~+ DC density was found in laryngeal carcinoma.An intensive VEGF expression is associated with the lower density DC.Conclusion The result suggests that the density of CD83~+ DCs in laryngeal carcinoma is negatively related to the expression of VEGF.更多还原