【摘要】 试验随机选取138头迪卡配套系猪B系、216头三江白猪和192头亚洲野猪提取基因组DNA进行聚合酶链式反应,得到RYR₁基因特异片段,经过双酶切鉴定,测得Hal^N基因频率分别为87．31%、92．13%、92．97%；Halⁿ的基因频率分别为12．69%、7．87%、7．03%。然后对3种氟烷基因型的猪进行肉质鉴定。更多还原

【Abstract】 138 DIKERS,216 white pigs,198 Sus scrofa were selected randomly,selecting blood samples from Sanjang White pigs,the DNA were distilled in blood anti purified,by PCR amplified and enzyme digestied,the genotypes of RYRI was analysised.The results indicated that:after conditioning optimized by PCR,a fragment of 659 bp was amplified by PCR,the fragment was digested by Hha l and Hgih I,three genotypes of Hal~NHal~N,Hal~N Hal~n,Hal~n Hal~n were gained.The frequency of HalN genotype among three species was 87.31%,92.13%,92.97% in turn.The frequency of Haln genotype was 12.69%, 7.87%,7.030%. (009)更多还原