【摘要】 根据绵羊、猪、小鼠和牛的Myostatin基因序列设计引物,通过PCR方法扩增了猪Myostatin基因的3′臂、5′臂,其长度分别为1.4 kb、4.4 kb。将扩增的片段与T载体连接后进行部分序列测定,与已发表的Myostatin序列进行同源性比较,同源性为100%;对目的片段与载体进行酶切后定向连接形成转基因结构,经序列测定后,证明其插入方向和位置完全正确,构建了猪的Myostatin表达Neor、Tk基因的双标记转基因载体。更多还原

【Abstract】 In this study,mammal double marker transgenic expression vector containing Neo~r gene and Tk gene was constructed.According to Myostatin gene sequence of the sheep,pig,mouse and cattle,we designed primers,and had gotten by PCR 3′-arm and 5′-arm of Myostatin gene of the pig which length were about 1.4 kb and 4.4 kb respectively.Amplified segments were connected with T-vector,and had portion sequencing.Then the sequence were compared with that had already published of Myostatin,and the homology was 100%.We had enzymed the objective segments on the vector,and then directional connected come into being transgenic structure.After sequencing,the inset direction and position was proved to be correct completely.更多还原