【摘要】 将提取的羊栖菜多糖用sevage法除蛋白、H2O2脱色后得到羊栖菜多糖半纯品,依次通过DE-AE-52、Sephadex G-200柱层析分离纯化后得到SFPS-B2、SFPS-C1、SFPS-D2三个多糖组分,用Sephacryl S200-HR柱层析鉴定纯度,三种组分的多糖为均一多糖,质量分数分别为86.02%、81.05%、86.95%.对SFPS-D2进行结构初步研究,通过紫外光谱、红外光谱和毛细管电泳分析,结果表明SFPS-D2不含蛋白质和核酸,多糖组成以β构型的吡喃糖苷键为主,含有硫酸基-O-SO3,其单糖组成以木糖、葡萄糖和半乳糖为主,其比例为木糖∶葡萄糖∶半乳糖=3.45∶5.8∶1.更多还原

【Abstract】 To obtain semi-purified polysaccharides from sargassum fusiforme by sevage’s method and H₂O₂.The semi-purified polysaccharides is further purified by loading on DEAE-52 and Sephadex G-200 respectively.Three groups（SPFS-B2,SPFS-C1,SPFS-D2） is obtained and proved to be homogeneous polysaccharides by Sehacryl S200-HR.The determination of polysaccharides was 86.02%,81.05%,86.95%.The SFPS-D2 primary structure was studied by UV,IR and HPCE.The results showed that the SFPS-D2 was mainly linked by β-glycosidics in its back bone and mainly composed of xylose,glucose,galactose（the ratio is 3.45∶5.8∶1）and sulfate（-O-SO₃） protein and nucleic acid is not found in SPFS-D2.更多还原