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羊栖菜多糖提取与含量的测定
Extraction and determination of polysaccharide from Sargassum fusiforme
【摘要】 通过水提醇沉方法提取羊栖菜粗多糖,纯化后测定羊栖菜中多糖含量.采用分光光度法、苯酚-硫酸显色法在490 nm处测定其含量.羊栖菜中多糖含量达53.46%,平均加样回收率为99.37%.该方法实验操作简便,准确可靠.
【Abstract】 To detect the content of polysaccharide frome Sargassum fusiforme,UV-spectrophotometer and phenol-sulfuric method were used,the detection wavelength was 490 nm.The percent of polysaccharide in Sargassum fusiforme was 53.46% and average recovery was 99.37%.This method is simple,convenient and reliable.
【关键词】 羊栖菜;
多糖;
含量测定;
硫酸苯酚法;
【Key words】 Sargassum fusiforme; polysaccharide; determination; phenol-sulfuric acid method;
【Key words】 Sargassum fusiforme; polysaccharide; determination; phenol-sulfuric acid method;
【基金】 国家自然科学基金资助项目(30271598)
- 【文献出处】 哈尔滨商业大学学报(自然科学版) ,Journal of Harbin University of Commerce(Natural Sciences Edition) , 编辑部邮箱 ,2006年01期
- 【分类号】R284
- 【被引频次】11
- 【下载频次】423