【摘要】 根据GenBank中发表的GPVB株全基因序列,应用生物学软件Primer5.0设计了针对鹅细小病毒VP3(574bp)的特异性引物,建立了小鹅瘟PCR诊断方法。对鹅细小病毒疫苗株和临床病料样品进行了PCR检测,结果从疫苗株和临床病料样品(7/9)中扩增到与预计大小一致的目的片段,而对照的鹅副粘病毒、新城疫病毒和鸭瘟病毒PCR结果均为阴性,敏感性检测结果表明,该PCR可以检测到0.124ng/L的GPV的核酸模板,因此,所建立的PCR方法特异性强,可用于临床病料的快速诊断。来源于黑龙江不同地区的送检样品检测结果表明,小鹅瘟分布广泛,仍是危害雏鹅的重要病毒性传染病。更多还原

【Abstract】 According to the gene sequence of GPV B strain of GenBank, a pair of specific primers were designed by Premier 5.0 to amplify VP3 gene from GPV by PCR.GPV PCR diagnostic methods was established to detect GPV vaccine strain and clinical samples. The results of PCR showed that the size of amplified products from vaccine strain and clinical samples(7/9) was 574bp. Nevertheless, GPMV, NDV, DPV were all negative, and as little as 0.124 ng/L of GPV could be detected in this PCR. The established PCR method was specific and could be used for rapid diagnosis of clinical disease. PCR testing of Heilongjiang samples showed that GP was widely distributed and continued to be an important contagious diseases.更多还原