【摘要】 选用低能(30keV)N+离子注入阿维拉霉素产生菌SV-56,研究其诱变效应。试验结果表明:SV-56菌株存活率曲线是典型的“马鞍型”剂量-效应曲线,“马鞍型”区域内具有高的正突变率(20．9％-26．2％)。通过5×1015cm-2N+注入诱变处理、链霉素抗性筛选,最终获得一株稳定性良好、阿维拉霉素产量稳定在79．6-82．9 mg·L-1之间、较出发菌株提高41．4％-47．2％的突变株SVN-116。更多还原

【Abstract】 Mutagenic effects of avilamycin-producing strain induced by N+ ion implantation have been investigated. The experimental results indicated that the survival rate curve took a "saddle" shape and the unique mutation effects of N+ ion implantation were presented. To obtain high-yield avilamycin-producing strain, the ion implantation dose of 5×1015 cm-2(the mutation rate was 20.9%-26.2%) was used and screening of streptomycin-resistant mutant was done in the study on breeding mutation. The SVN-116 mutant was obtained, with an avilamycin yield of 79.6-82.9 mg·L-1 that was 1.414-1.472 times as much as the original strain.更多还原