【摘要】 从农药厂废水处理池的活性污泥中分离到1株能以甲氰菊酯为唯一碳源生长的细菌,命名为JQL4-5.根据其生理生化特征和16S rDNA(GenBank Accession No.DQ177525)序列相似性分析,将该菌株鉴定为鞘氨醇单胞菌属(Sphingomonassp.).该菌株在24h内对20 mg/L的甲氰菊酯的降解率达到99.8%.降解甲氰菊酯的最适温度为30℃,pH为7.0,降解速率与初始接种量呈正相关.酶的定域试验表明,降解甲氰菊酯的酶为胞内酶.更多还原

【Abstract】 A bacterium capable of utilizing fenpropathrin as sole carbon source was isolated from activated sludge collected from wastewater-treating system of a pesticide manufacturer.This bacterium was identified as Sphingomonas sp.according to its physiological & biochemical analysis and the similarity analysis of its 16S rDNA sequence(GenBank Accession No.DQ177525).This bacterium could degrade 99.8% of(20mg/L) fenpropathrin in 24h.The optimal pH and temperature for the degradation were 7.0 and 30℃,respectively.The degradation speed was related positively to initial inoculum size.The enzyme distribution experiment showed that the degrading-enzyme in the bacterium was endoenzyme.更多还原