【摘要】 从北京某避孕药厂污水处理站曝气池的活性污泥中分离得到1株能够以17α-乙炔基雌二醇(17α-ethinylestradiol,EE2)为唯一碳源和能源生长的菌株JCR5.通过对其形态特征、生理生化以及16S rDNA序列分析,鉴定该菌株为鞘氨醇杆菌属(Sphingobacteriumsp.).菌株JCR5在10d内对初始底物浓度为30 mg.L-1EE2的降解率可达87%.该菌株能够利用甾体雌激素(甾酮、17β-雌二醇、雌三醇以及炔雌醇甲醚)、避孕药生产中间体以及一些芳烃化合物为唯一碳源生长.对EE2降解中间产物进行质谱分析推测,EE2在降解过程中首先被氧化为雌酮(estrone,E1),后经过一系列生物催化作用生成2-羟基-2,4-二烯-戊酸和2-羟基-2,4-二烯-1,6-己二酸2种中间代谢产物.前者是与Comamonas testosteroniTA441代谢睾甾酮的途径类似的产物,而后者是3-羟基-4,5-9,10-二断雌甾烷-1(10),2-二烯-5,9,17-三酮基-4-酸不同于前者断键位置的另一产物.更多还原

【Abstract】 A bacterial strain,JCR5,which degrades 17α-ethynylestradiol(EE2),was isolated from activated sludge of wastewater treatment plant treating wastewater from pharmacy factory mainly producing contraceptive medicine in Beijing,China.Based on its morphology,physiology biochemical properties and 16S rDNA sequence analysis,this strain was identified as Sphingobacterium sp.JCR5.Strain JCR5 can grow on EE2 as sole carbon and energy source.The degradation process for EE2 with initial concentration of 30 mg·L~-1 was studied,and the result indicated that the degradation rate of EE2 within 10 days was 87%.Experiments of different substrates showed that strain JCR5 can grow on many substrates other than EE2 such as several steroidal estrogens(estrone,17β-estradiol,estriol and mestranol),the intermediates in contraceptive medicine processing and some aromatic compounds.Mass spectrum analysis demonstrated that EE2 was oxygenized to estrone(E1) firstly,and 2-hydroxy-2,4-dienevaleric acid and 2-hydroxy-2,4-diene-1,6-dioic acid were the main catabolic intermediates during EE2 degradation.The former adopted a pathway that was analogical to the pathway of the previously reported testosterone-degrading Comamonas testosteroni TA441,and the latter was a metabolite with a different cleavage position of 3-hydroxy-4,5-9,10-disecoestrane-1(10),2-diene-5,9,17-trione-4-oic acid from the former.更多还原