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兼性厌氧苯胺降解菌的分离鉴定及其特性
Isolation and Characterization of a Facultative Anaerobic Aniline-Degrading Bacterium
【摘要】 从处理印染废水的厌氧折流板反应器(ABR)系统中分离、纯化并筛选出1株能以苯胺为唯一碳氮源进行代谢的兼性厌氧苯胺降解菌株AN29.经过形态、生理生化特征试验和16S rDNA序列分析结果,鉴定菌株AN29为假单胞菌(Pseudomonassp.),其特性为:降解苯胺的最适温度为37℃,降解苯胺合适的起始pH值为6.5~8.0,可以利用苯胺的最高浓度为4 000mg/L,合适起始浓度为500~2 000mg/L.
【Abstract】 An aniline-degrading bacterium(designated strain AN29) was isolated from dyeing wastewater process(anaerobic baffled reactor,ABR) with the capability of utilizing aniline as sole carbon source and nitrogen source.It was identified as Pseudomonas sp.based upon the phenotypic properties and a partial analysis of the 16S rDNA.The strain could degrade aniline under the aerobic and anaerobic conditions,the optimal initial pH 6.5~8.0,a temperature of 37℃,and initial aniline concentrations of 500~(2?000)(mg/L) with maximum concentration of(4?000)(mg/L) respectively.
【Key words】 aniline; facultative anaerobic; biodegradation; characterization;
- 【文献出处】 环境科学 ,Environmental Science , 编辑部邮箱 ,2006年08期
- 【分类号】X172
- 【被引频次】23
- 【下载频次】438