【摘要】 研究了水解淀粉乳酸细菌对厨余垃圾发酵生产乳酸的强化作用.从厌氧发酵的厨余垃圾中分离到6株水解淀粉的乳酸细菌,其中菌株FH164具有最高的淀粉降解率和乳酸产量.在pH5.5~6.0条件下,经48h发酵,菌株FH164从40.50g.L-1的可溶性淀粉产生32.67g.L-1的乳酸.根据形态、生理生化特征和16SrDNA序列分析结果,可将菌株FH164鉴定为链球菌属(Streptococcussp.)细菌.接种菌株FH164可强化厨余垃圾的乳酸发酵,采用垃圾不灭菌的开放式发酵,菌株FH164可得到28.23g.L-1的乳酸,比自然发酵(不接种的对照)的乳酸浓度高19.2%.更多还原

【Abstract】 In order to realize resource recycling of kitchen waste,the enhancement of fermentative production of lactic acid（LA） by a strain of amylolytic lactic acid bacterium（ALAB） isolated from kitchen waste was investigated.A total of 6 strains of ALAB were isolated from anaerobically fermented kitchen waste.Among these isolates,the strain FH164 exhibited the highest starch-hydrolyzing rate and LA production.32.67g·L^-1 of LA was produced from 40.50g·L^-1 of soluble starch by strain FH164 at pH 5.5～6.0 in 48h of fermentation.On the basis of its morphological and physio-biochemical characteristics as well as 16S rDNA sequence,the strain FH164 was tentatively identified as Streptococcus sp..28.23g·L^-1 of LA was obtained from kitchen waste by the strain FH164 employing an open fermentation mode（substrate non-autoclaved）,which was 19.2% higher than that of the fermentation without inoculum（control）.更多还原