【摘要】 以一株降解甾体雌激素17α-乙炔基雌二醇(EE2)的鞘氨醇杆菌(Sphingobacteriumsp.)JCR5为材料,研究了JCR5降解EE2的适宜接种量、底物利用情况和添加营养物对降解能力的影响.结果表明,在接种量为7%(体积分数)(OD600≈1)时,EE2的降解率最高;JCR5能够利用甾体雌激素(甾酮、17β-雌二醇、雌三醇以及炔雌醇甲醚),避孕药生产中间体以及一些芳烃化合物为唯一碳源生长;添加少量葡萄糖、蛋白胨和酵母粉,均能促进JCR5对EE2的降解.葡萄糖、17β-雌二醇(E2)分别与EE2的共基质代谢试验表明,在共基质条件下,葡萄糖的存在对EE2的降解起到一定的促进作用,而E2的存在对EE2的降解具有一定的抑制作用.利用HPLC检测EE2降解过程发现,在EE2降解的同时伴有未知的EE2代谢产物峰出现.更多还原

【Abstract】 Using a strain of 17α-ethinylestradiol（EE2）-degrading Sphingobacterium sp.JCR5 as material,the optimal inoculating dose,different substrates,and added nutrition for JCR5 to degrade EE2 was studied.It was shown that when inoculating 7% of bacterium solution with OD₆00 of 1,the highest degrading rate of EE2 was obtained.The strain JCR5 can grow on many substrates other than EE2 such as several steroid estrogens（estrone,17β-estradiol,estriol and mestranol）,intermediate chemicals in contraceptive medicine processing and some aroma chemicals.Adding little amounts of glucose,tryptone and yeast extract can promote its degradation of EE2.Co-metabolism experiments showed that the existence of glucose can promote the degradation of EE2 and the existence of E2 can inhibit the degradation of EE2.The process of degradation of EE2 was tested by HPLC,and it should be noted that there were unknown products of EE2-metabolism.更多还原