【摘要】 为验证气提式三重循环生物膜反应器处理甾体雌激素的效能,建立了一套SPE/HPLC/MS/MS分析方法,对反应器进、出水中的甾体雌激素———甾酮(E1),17β-雌二醇(E2),雌三醇(E3)以及17α-乙炔基雌二醇(EE2)进行检测.该方法的加标回收率为88%~103%,精密度为4%~9%.该方法对E1,E2,E3和EE2的进水定量限(LOQ)分别为0.7,0.8,0.9和0.5 ng/L;对出水的定量限分别为2.0,1.0,2.0和1.0 ng/L.CODCr和氨氮容积负荷最高为7.5和1.6 kg/(m3.d),出水依然能够保持稳定.进水pH稳定在9.0~10.5,而反应器的pH一直稳定在9.0以下,体系对pH变化造成的冲击比较适应.同时,反应器内形成了稳定的NO2--N积累,CODCr和氨氮的去除率分别达到了70%和73%.更多还原

【Abstract】 An analytical method performed by solid-phase extraction（SPE） system with high pressure liquid chromatography and tandem mass spectrometry（HPLC/MS/MS） was developed for validating the efficiency of the triplet loop airlift biofilm reactor（TLABR）.The steroid estrogens,including estrone（E1）,17β-estradiol（E2）,estriol （E3） and 17α-ethynylestradiol（EE2）,of influent and effluent of the bioreactor were determined by this method.The average recoveries ranged from 88% to 103% and the precision of the method ranged from 4% to 9%.The limit of quantification（LOQ） was determined at 0.7 ng/L for E1,0.8 ng/L for E2,0.9 ng/L for E3,and 0.5 ng/L for EE2 in influent,and 1.0 ng/L for E2 and EE2,and 2.0 ng/L for E1 and E3 in effluent.The effluent could still remain stable,when the influent volume load of COD_Cr and NH₃-N reached as high as 7.5 and 1.6 kg/（m³·d） respectively.The influent pH was generally kept at 9.0 ～10.5,and the pH within the reactor remained stable at below 9.0.Therefore,the system could adapt to the impact of pH.Also,stable accumulation of NO₂^——N was formed in the reactor,and the removal rates of COD_Cr and ammonia reached 70% and 73%,respectively.更多还原