【摘要】 利用热带假丝酵母原始菌F10-1及已构建的肉毒碱乙酰转移酶基因单拷贝缺失重组菌CZ-15,在5L发酵罐中进行了补糖发酵批式培养,生产十三碳二元酸(DCA13).结果表明,肉毒碱乙酰转移酶基因单拷贝缺失后,CZ-15菌株的最佳补糖时间和补糖浓度都与原始菌F10-1不同.采用补糖工艺后,F10-1与CZ-15的十三碳二元酸的产量分别提高了25.0%和23.4%,达到99.5和108.1g/L,烷烃转化率分别提高了35.5%和28.6%.更多还原

【Abstract】 Candida tropicalis strain F10-1 and its recombinant strain CZ-15 can be used for 1,11-dicarboxylic acid(DCA13)production,and in CZ-15,one copy of carnitine acetyltransferase(CAT)genes is disrupted.The sucrose-added fed-batch fermentation of F10-1 and CZ-15 was studied.The results showed that the optimal feeding time and the optimal sucrose concentration were different for F10-1 and CZ-15.By feeding sucrose in the acid production stage,for F10-1 and CZ-15,the acid production was increased by 25.0% and 23.4%,reaching 99.5 and 108.1 g/L,and the alkane conversion rate was increased by 35.5% and 28.6%,respectively.更多还原