【摘要】 采用微孔膜乳化法与复乳法结合制备粒径均一可控的以聚乳酸和聚(乳酸-羟基乙酸)共聚物为膜材的载溶菌酶微胶囊,粒径分布系数CV(CoefficientofVariation)为14.04%,远低于机械搅拌法制备的微囊的CV(76.54%).分别加入内水相添加剂PVA,PEG400,HP-β-CD,使溶菌酶的包埋率从无添加剂时的68.1%分别增大到86.6%,89.0%和94.1%.添加剂降低了溶菌酶的突释.PEG400,PEG6000,HP-β-CD的加入降低了溶菌酶的释放速率,而PVP或PVA的加入则加快了溶菌酶的释放.溶菌酶在油水界面上的吸附变性是失活的主要原因.在酶液中加入PEG400,PEG6000,PVP,HP-β-CD可有效地避免由于油水界面造成的溶菌酶活性的损失.更多还原

【Abstract】 Relatively uniform-sized biodegradable poly(lactide)(PLA)and poly(lactide-co-glycolide)(PLGA)microcapsules containing lysozyme were successfully prepared by combining porous glass membrane emulsification technique with multiple emulsion-solvent evaporation method.Compared with the stirring method,it was found that the sizes of microcapsules are more uniform,the coefficient of variation(CV)value which indicates the size distribution was 14.04%.It was found that adding additives to inner aqueous phase could efficiently improve the drug encapsulation efficiency,adjust the drug release profile and maintain the bioactivity of drug.When PVA,PEG400 and HP-β-CD were separately added into the inner aqueous phase,the encapsulation efficiency increased from 68.1% to 86.6%,89.0% and 94.1% respectively.The cumulative release amount was decreased when PEG400,PEG6000,HP-β-CD were added.While it was enhanced when PVP or PVA was added.It demonstrated that the main reason for protein deactivation was lysozyme adsorption onto the W1/O interface.The loss of lysozyme activity was successfully prevented by adding PEG400,PEG6000,HP-β-CD or PVP into the inner aqueous phase.更多还原