【摘要】 利用温控载体pHsh将编码甘油脱水酶的基因dhaB和编码1,3-丙二醇氧化还原酶同工酶的基因yqhD串联构建重组质粒pHsh-dhaB-yqhD,将其转化大肠杆菌得到新型产1,3-丙二醇重组大肠杆菌JM109(pHsh-dhaB-yqhD),并对影响该重组菌发酵的营养因子进行研究.实验结果表明:该重组菌转化甘油合成1,3-丙二醇的适宜培养基组成为甘油60g·L-1、酵母膏5·0g·L-1、维生素B120·05g·L-1以及KH2PO47·5g·L-1;以此培养基在5L发酵罐上进行放大,1,3-丙二醇产量、转化率和生产能力分别达到43·26g·L-1、72·2%和1·55g·L-1·h-1.更多还原

【Abstract】 The gene dhaB encoding glycerol dehydrase from Citrobacter freundii gene dhaB and 1,3-propanediol （1,3-PD）oxidoreductase isoenzyme encoding gene （yqhD） were transferred into temperature control expression vector pHsh to construct a novel recombinant Escherichia coli JM109 （pHsh-dhaB-yqhD）.Some nutrient ingredients affecting fermentation results of this recombinant strain were also studied.The results demonstrated that the optimized medium comprised（g·L -1 ） glycerol 60, yeast extract 5.0, KH₂PO₄ 7.5 and vitamin B 12 0.05.The 1,3-propanediol concentration, yield on glycerol and productivity in the 5 L fermenter could reach 43.26 g·L -1 , 72.2 % and 1.55 g·L -1 ·h -1 respectively.更多还原