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介孔分子筛SBA-15的表面改性对脂肪酶固定化的强化作用

Improvement of lipase immobilization by surface modification of SBA-15 mesoporous silica

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【作者】 徐坚杨立明王玉军骆广生戴猷元

【Author】 XU Jian,YANG Liming,WANG Yujun,LUO Guangsheng,DAI Youyuan (State Key Laboratory of Chemical Engineering,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)

【机构】 清华大学化学工程系化学工程联合国家重点实验室清华大学化学工程系化学工程联合国家重点实验室 北京100084北京100084

【Abstract】 Three kinds of support for enzyme immobilization were prepared by modifying SBA-15 mesoporous silica with thiol,methyl and hexyl groups respectively.The prepared supports were characterized with FT-IR and BET method and exhibited high specific surface area and uniform pore size.Furthermore,unmodified SBA-15 and the modified SBA-15 materials were used for lipase immobilization.The results were compared in terms of enzyme adsorption capacity,activity,activity retention and stability.The modified SBA-15 materials with thiols(SBA-15-PrSH)showed good performance for lipase immobilization.Compared with unmodified SBA-15,the adsorption capacity of SBA-15-PrSH to lipase was improved by more than 80%,and the activity retention increased by more than 10%.

【关键词】 介孔分子筛SBA-15脂肪酶固定化
【Key words】 mesoporousSBA-15lipase immobilization
【基金】 国家自然科学基金项目(20206012).~~
  • 【文献出处】 化工学报 ,Journal of Chemical Industry and Engineering(China) , 编辑部邮箱 ,2006年10期
  • 【分类号】TQ28
  • 【被引频次】31
  • 【下载频次】657
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