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Mutagenesis and selection of high efficiency hydrogen-producing mutants by ultraviolet radiation

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【作者】 任南琪郑国香李永峰林海龙李建政

【Author】 REN Nan-qi, ZHENG Guo-xiang, LI Yong-feng, LIN Hai-long, LI Jian-zheng (School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090, China)

【机构】 School of Municipal and Environmental Engineering Harbin Institute of TechnologySchool of Municipal and Environmental EngineeringHarbin Institute of TechnologyHarbin 150090 China

【Abstract】 Hydrogen is an ideal, clean and sustainable energy source for the future because of its high conversion and nonpolluting nature. Biohydrogen production by dark-fermentation appears to have a great potential to be developed for practical application. However, one limiting factor affecting the development of hydrogen-production industrialization is that the hydrogen-producing capacity of bacteria is lower, so how to increase bacteria’s hydrogen-producing ability will be an urgent issue. In this experiment, 2 mutants, namely UV3 and UV7, were obtained by ultra-violet radiation. They grew and produced hydrogen efficiently on iron-containing medium. The hydrogen evolution of UV3 and UV7 were 2 356.68 ml/L and 2 219.62 ml/L at a glucose concentration of 10 g/L, respectively. With wild parent strain Ethanoligenens sp. ZGX4, the hydrogen evolution was 1 806.02 ml/L under the same conditions. Mutants’ hydrogen-producing capacities were about 29.71% and 22.22% higher than that of wild parent strain ZGX4. The maximum H2 production rate by mutants UV3 and UV7 were estimated to be 32.57 mmol H2/g cell h and 31.19 mmol H2/g cell h, respectively, which were 38.18% and 34.78% higher than the control (23.57 mmol H2/g cell h). The abundant products of UV3 and UV7 were ethanol and acetic, which accounted for 95%-98% of total soluble microbial products. In each case, mutant strains UV3 and UV7 evolved hydrogen at a higher rate than the wild type, showing a possible potential for commercial hydrogen production. Another mutant named UV20’ was also gained whose main end metabolites were butyric acid and acetic acid. This would provide researched material for a discussion of metabolic pathways of hydrogen-producing bacteria.

【关键词】 biohydrogenUV-mutagenesismutants
【Key words】 biohydrogenUV-mutagenesismutants
【基金】 Sponsored by“973”Fundamental Science Program of China(Grant No. G2000026402) and National Natural Science Fund of China (Grant No. 30470054).
  • 【文献出处】 Journal of Harbin Institute of Technology ,哈尔滨工业大学学报(英文版) , 编辑部邮箱 ,2006年06期
  • 【分类号】X172
  • 【被引频次】2
  • 【下载频次】146
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