【摘要】 为了进一步研究苏丹Ⅰ、Ⅲ、Ⅳ对人胃癌细胞(SGC-7901)增殖的影响,采用流式细胞仪测定了DNA含量的变化,分析细胞周期的变化.结果表明:苏丹Ⅰ、Ⅲ、Ⅳ促进了SGC-7901细胞由G1期进入S期和G2期,表现为G1期细胞平均DNA含量下降,S期DNA含量上升,G2期DNA含量上升,细胞增殖指数(PI)增加,S期细胞指数(SPF)增加.其中苏丹Ⅲ在50μg.mL-1剂量时对SGC-7901细胞增殖影响非常显著(P<0.01),苏丹Ⅰ、Ⅳ在10μg.mL-1剂量时对SGC-7901细胞增殖影响非常显著(P<0.01).苏丹Ⅰ、Ⅲ、Ⅳ是通过影响细胞周期,从而调节SGC-7901细胞的增殖活动,促进细胞分化增殖.更多还原

【Abstract】 Objective to study the effect of SudanⅠ,Ⅲ,Ⅳ on the growth of SGC-7901.The change of DNA content is determined with Flow Cytometry,and the cell cycle is analyzed.Results show that the average content of DNA decreases in G₁ phase and increases in S and G₂ phase.The index of PI and SPF increase.It is indicated that SudanⅠ,Ⅲ,Ⅳ accelerate the cell cycle from G₁ phase to S and G₂ phase.It is also shown that the effects on SGC-7901 by Sudan Ⅲ in the dose of 50 μg·mL^-1 and by SudanⅠ,Ⅳ in the dose of 10μg·mL^-1 is very effective（P<0.01）.It is concluded that SudanⅠ,Ⅲ,Ⅳ affect SGC-7901 cell cycle to advance its differentiation and proliferation.更多还原