【摘要】 通过二维电泳(2-DE)技术来研究脉冲电场作用后48h中国红豆杉细胞在蛋白表达上的变化．共进行了3次二维电泳重复实验,经过相关因子分析证实具有较高的重复性．比较对照组和经过脉冲电场处理的实验组的二维电泳图谱,发现有较明显的差异．用基质辅助激光解析-飞行时间质谱和液相色谱-电喷雾质谱对其中的部分蛋白进行分析鉴定．鉴定结果表明：核糖核酸蛋白粒、醛糖差向异构酶和铁氧还蛋白-NADP(+)还原酶这三个蛋白可能是与脉冲电场诱导相关的蛋白．更多还原

【Abstract】 Two dimensional gel electrophoresis(2-DE) was used to analyse changes of the protein ex- pression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field(PEF)elicitation. The 2-DE experiments were repeated for 3 times,and proved to be reliable according to the correlation coefficient analysis.Distinct changes were found from comparison of the protein patterns of control and PEF treated cells.Matrix-assisted laser desorption/ionization mass spectrometry and electrospray ioni- zation mass spectrometry were used to analyse some proteins.The results indicated that heterogeneous nuclear ribonucleoprotein(hnRNP),aldose l-epimerase family protein and Ferredoxin-NADP(+)re- ductase,putative/adrenodoxin reductase are related to the induction of PEF.更多还原