【摘要】 报道了通过毛细管电泳-安培检测法测定腺嘌呤(A)和鸟嘌呤(G)来间接测定酵母菌中核糖核酸含量的方法.探讨了电极电位、运行缓冲溶液、分离电压及进样时间等对毛细管电泳分离-安培检测的影响,得到了较为优化的测定条件.在30 mmol/L的硼酸盐缓冲液(pH 9.0)中,上述两组分可在18 min内完全分离.腺嘌呤和鸟嘌呤的检测限分别为2.0×10-7mol/L和1.7×10-7mol/L.该法具有较好的重现性和灵敏度,可以用于食品中酵母菌中核糖核酸的快速测定.更多还原

【Abstract】 The indirect determination of the content of RNA in yeast by analyzing adenine（A） and guanine（G） with capillary electrophoresis with amperometric detection（CE-AD） was reported.The effects of potential of working electrode,running buffer,separation voltage and injection time on the CE-AD determination were investigated.Adenine and guanine could be well separated in a 30 mmol/L borate buffer（pH 9.0） within 18 min under the optimum conditions.Their detection limits of adenine（S/ N = 3） of 2.0 ×10 ^-7 mol/ L and 1.7 ×10 ^-7 mol/ L,respectively.The experimental results showed that this method is of high sensitivity and high repeatability,and potential to be used in the quick analysis of yeast in foods.更多还原