【摘要】 目的探讨孕酮对大鼠局部脑缺血再灌注损伤的神经保护作用。方法以栓线法制备大脑中动脉梗阻脑缺血再灌注大鼠模型,利用TTC和免疫组织化学染色法分别观察缺血面积大小和c-fos在大脑皮质的表达。结果①缺血再灌注组脑缺血面积明显大于缺血组(P<0.05);各实验组不同时间给予孕酮后脑梗死面积明显小于相应对照组(P<0.05)。②缺血组和缺血再灌注2h组可见Fos阳性细胞主要分布于缺血区周围的半影区;各实验组不同时间给药后扣带回皮质Fos阳性细胞数均高于相应对照组(P<0.05)。结论孕酮对大鼠局部脑缺血和再灌注具有明显的神经保护作用。更多还原

【Abstract】 ObjectiveThe neuroprotective effects of progesterone was evaluated in rats with middle cerebral artery occlusion(MCAO) and reperfusion. MethodsThe rats model of MCAO and reperfusion was produced with a string that was inserting into the internal carotid artery to occlude MCA. TTC staining and immunocytochemistry were used to analyze infarction size and c-fos expression in region arounding infarction area. Results①The infarction size in rats with MCAO and reperfusion was significantly larger than that in rats with MCAO without reperfusion(P< 0.05 ). The infarction size in rats with MCAO pretreated with progesterone were all smaller than that in the ischemic rats in corresponding time points. ②c-fos expression was mainly distributed in ischemic penumbra with MCAO combined with or without reperfusion. The numbers of Fos-positive neurons with pretreatment of progesterone were larger than those in the ischemic rats significantly(P< 0.05 ). ConclusionThe results may suggest that progesterone has some neuroprotective effects to MCAO rats whether combined with reperfusion or not.更多还原