【摘要】 目的:研究乌司他丁抗衰老的作用。方法:建立小鼠衰老模型,随机分为对照组及治疗组,分别腹腔注射生理盐水及乌司他丁。3周后,采用ELISA法检测小鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、IL-6、IL-8的含量;称重检测胸腺指数(TI)和脾脏指数(SI);采用流式细胞术检测CD4+T与CD8+T细胞。结果:乌司他丁治疗组小鼠血清SOD的含量升高,MDA的含量下降;血清IL-6、IL-8的含量降低;胸腺指数升高而脾脏指数无明显改变;血液中CD4+T细胞数量未改变,而CD8+T细胞数量升高,CD4+T细胞与CD8+T细胞的比值降低。结论:乌司他丁可抵抗自由基及其代谢产物引起的脂质过氧化,可延缓胸腺萎缩,增加T细胞的数量,提示其具有一定的抗衰老作用。更多还原

【Abstract】 Objective: To study the anti-aging role of ulinastatin in mice.Methods: The senescent mice models were made and divided randomly into control group and treatment group,and were injected with normal sodium and ulinastatin respectively.After three weeks,the levels of SOD,MDA,IL-6 and IL-8 in the mice serum were detected by ELISA.The TI and SI were measured,and CD4⁺T cells and CD8⁺T cells were counted by flow cytometry.Results: In the treatment group,the level of SOD in the serum of mice was highly increased and the levels of MDA,IL-6 and IL-8 were decreased.The TI was increased,while the SI never changed.The amount of CD4⁺T cells did not change significantly,while the amount of CD8⁺T cells was increased and the ratio of CD4⁺/CD8⁺T cells were decreased.Conclusion: Ulinastatin might resist the lipid peroxidation which was caused by free radicals and its products of metabolism,might delay the process of atrophy of thymus gland,and increase the amount of T cells as well.The experiment showed that the ulinastatin might have the function of anti-aging.更多还原