【摘要】 目的:探讨PC12细胞缺氧缺糖损伤中,单唾液酸四己糖神经节苷脂(GM1)对血红素加氧酶(HO-1)表达的影响,并研究PI-3K/Akt通路在此过程中的作用。方法:用连二亚硫酸钠(Na2S2O4)消除培养基中的氧合并培养基质缺糖制造PC12细胞缺氧缺糖损伤(OGD)模型,用RT-PCR和Western blot方法检测不同剂量(25-100μmol/L)GM1和PI-3K抑制剂LY294002对缺氧缺糖损伤时PC12细胞HO-1mRNA及蛋白表达的影响。结果:与对照组和单纯缺氧缺糖组相比,GM1可显著上调缺氧缺糖PC12细胞的HO-1mRNA和蛋白表达,且两者呈明显的剂量依赖关系;PC12细胞经LY294002预处理后,GM1上调HO-1mRNA和蛋白表达的作用均受到抑制。结论:GM1可上调缺氧缺糖损伤时PC12细胞HO-1mRNA和蛋白表达,PI-3K/Akt通路在此过程中发挥了一定的信号转导作用。更多还原

【Abstract】 Objective: To investigate the effects of Monosialoganglioside（GM1）on the heme oxygenase-1（HO-1）expression in PC12 cells during oxygen glucose deprivation（OGD）,and to study the role of phosphatidylinositol 3-kinase（PI-3K）/Akt signaling pathway in this course.Methods: An ischemical model was induced by incubation of PC12 cells with 1 mmol/L Na₂S₂O₄ and glucose-free medium for four hours.Cells were treated with differect dose（25-100 μmol/L）of GM1 at the same time and（or）pretreated with LY294002,an inhibitor of PI-3K during OGD.Expression of HO-1mRNA and protein was determined by RT-PCR and Western blot analysis.Results: In contrast with the control group and OGD group,GM1 could up-regulate markedly the expression of HO-1mRNA and protein during OGD.Moreover,there was characteristic dose-depended relationship between GM1 and the expression of HO-1 mRNA and protein.Pretreatment of cells with LY294002 could partially attenuated the ability of GM1 to up-regulate HO-1mRNA and protein expression.Conclusion: GM1 could up-regulate HO-1 mRNA and protein expression in PC12 cells during OGD,and PI-3K/Akt signaling pathway plays an important role in this course.更多还原