【摘要】 目的:明确盐酸阿比朵尔抗吸道合胞病毒(RSV)Long株的活性并探讨其抗病毒机制。方法:通过细胞病变效应(CPE)、噻唑蓝(MTT)比色法检测细胞活性,观察盐酸阿比朵尔抗RSV的作用。结果:盐酸阿比朵尔的半数毒性浓度(TD50)为85.39 mg/L,当该药浓度为25 mg/L时,药物抗病毒生物合成组、药物直接作用组、药物抗吸附4 h组、药物抗病毒吸附8 h组的病毒抑制率分别能达到81.61%,79.57%,40.39%和78.60%。4组相应的药物半数有效浓度(ED50)为11.52,8.74,25.36和10.40 mg/L。结论:盐酸阿比朵尔在体外能明显抑制RSV引起的CPE效应,病毒抑制率随药物浓度的增加而增高,盐酸阿比朵尔能通过多种途径发挥抗RSV的作用。更多还原

【Abstract】 Objective: To study the antiviral activity of Arbidol hydrochloride on respiratory syncytial virus（RSV） Long strain in vitro.Methods: Anti-RSV effects were investigated by observing cytopathic effect（CPE） with MTT colorimetric assay.Results: The median cell inhibitory(TD₅₀) of Arbidol was 85.39 mg/L and at its concentration of 25 mg/L, the viral inhibitory rates were up to 81.61%,（79.57%,） 40.39% and 78.60% respectively in antiviral biological synthesis group,directly-killing-virus group and interfering-in-viral-absorption groups（pretreated cells with Arbidol for four and eight hours respe-ctively）.The corresponding median efficacious(EC₅₀) concentrations of Arbidol（against） RSV in the four groups were 11.52,（8.74,）（25.36） and 10.40 mg/L,respectively.（Conclusion:） Arbidol could significantly inhibit cytopathic effect（CPE） of RSV-infected HEp-2 cells and increase survival rates of RSV-infected cells in a dose dependent manner,and could be used as an effective drug for RSV infection.更多还原