【摘要】 以MS培养基为基础,分别以甘草根、胚轴、子叶为外植体进行培养,考察光照2、,4 D质量浓度、不同激素组合、pH等理化因子对愈伤组织生长的影响,对代谢产物甘草酸进行了HPLC的定量测定.实验表明其中以根的愈伤组织中甘草酸含量最高,为70.2 mg/g;黑暗条件比光照条件更有利于甘草酸合成;以激素组合为2,4 D 4.0 mg/L+KT 0.5 mg/L,pH为5.4时甘草酸含量最高,在此条件下悬浮培养根培养物中甘草酸质量分数为81.6 mg/g,比生药增多51.08%.更多还原

【Abstract】 Root,hypocotyl and cotyledon of Glycyrrhiza inflata were cultured on MS medium,the effects of some physico-chemical factors on the induction,culture of callus were also investigated,the content of glycyrrhizic acid in the cultures were showed.The results showed:the glycyrrhizic acid content of root cultures was 70.2?mg/g,obviously higher than that of other culture;the glycyrrhizic acid content was higher in dark than that in light;the glycyrrhizic acid content of root suspended culture was 81.6?mg/g,increase by 51.08?% than the crude drug,when hormone association was 2,4-D4.0?mg/L+KT 0.5?mg/L,pH=5.4.更多还原