【摘要】 以丝羽乌骨鸡为材料,在家鸡1号染色体和Z染色体上分别选取15对和5对微卫星标记,应用多重PCR技术对140只试验个体的基因组DNA进行了扩增,结果表明,所选用的20对微卫星引物中,除MCW0101和MCW0181因扩增效果不理想而剔除外,其余18对标记均表现出丰富的多态性,所有结果都能重复。18对微卫星引物平均检测到6.83个等位基因(5～8个),平均杂合度和多态信息含量分别为0.833和0.805。其结果可作为进一步QTL定位和标记辅助选择研究的参考。更多还原

【Abstract】 Fifteen microsatellite markers in Chr1 and five microsatellite markers in ChrZ were selected for multiplex PCR in 140 silky fowls.The results indicated that 18 pairs of microsatellites were polymorphism.The average number of alleles detected by a pair of primer was 6.83.The average PIC was 0.805.And the population heterozygosity was 0.833.更多还原