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苏云金芽孢杆菌“94001”菌株发酵工艺的优化
Study on The Fermentation Technology of Bacills Thuringiensis
【摘要】 苏云金芽孢杆菌“94001”菌株最佳发酵工艺条件是通气量10∶.5~1.2V.V-1.min-1,培养温度34℃,搅拌速度250r.min-1。经40m3发酵罐发酵试验,平均发酵效价为6484IU.μL-1,平均晶体蛋白含量为0.61%。
【Abstract】 The fermentation technology of Bacillus thuringiensis bacillin "94001" was studied in this paper.Experimental results of 2.3m3 fermenter showed that the most favorable ventilatation quantity was 1∶0.5~1.2,temperature was 34℃,rotating speed was 250r·min-1.By experimentation of 40m3 fermenter,the value of potency and crystal protein content reached 6 484IU·μL-1 and 0.61% respectively.
【关键词】 苏云金芽孢杆菌“94001”;
正交设计;
发酵水平;
【Key words】 Bacillus thuringiensis "94001"; orthogonal design; fermentation;
【Key words】 Bacillus thuringiensis "94001"; orthogonal design; fermentation;
【基金】 湖北省重点科技攻关项目(971PO712)
- 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2006年03期
- 【分类号】TQ453.5
- 【被引频次】5
- 【下载频次】229