【摘要】 本试验利用21个RAPD随机引物对50个西瓜枯萎病菌系进行了RAPD扩增,研究了病菌遗传多样性。结果表明,对供试菌系扩增出134条带,其中多态性带113条,占总带数的84.3%。菌系间的遗传相似系数变化在0.45~0.93之间,多数菌系间的同源程度较低。基于RAPD标记聚类分析表明,50个菌系划分为6个RAPD群(RAPDgroups,简称RGs),即RGⅠ,RGⅡ,RGⅢ,RGⅣ,RGⅤ和RGⅥ。其中RGⅠ和RGⅡ各含有一个菌系,分别来自秦皇岛和新疆;RGⅢ和RGⅤ包括2个菌系,分别来自秦皇岛、承德和廊坊、沧州;RGⅣ包括唐山、石家庄、邯郸和新疆菌系;其余的菌系属于RGⅥ。分类结果进一步说明西瓜枯萎病菌间存在着较大的遗传分化。更多还原

【Abstract】 Fifty isolates of Fusarium oxysporum f.sp niveum were RAPD analyzed by using 21 random primers.The result showed that 134 RAPD bands were amplified,among them,113(84.3%) bands were polymorphic.Genetic similarity coefficients within the isolates were among 0.45～0.93,there existed a low degree of homogeneity between most of them.When all of polymorphic bands were analyzed by cluster analysis,50 isolates were classified into 6 groups: Both RG Ⅰ and RGⅡ included 1 isolate,respectively,from Qinhuangdao and Xinjiang;RGⅢand RGⅤexisted 2 isolates,respectively,from Qinghuangdao,Chengde and Langfang,Cangzhou;RGⅣ,It concluded 5 isolates which came from Tangshan,Shijiazhuang,Handan and Xinjiang areas;the others isolates belonged to RGⅥ.The result further demonstrated that there existed greater genetic differentiation among watermelon wilt isolates.更多还原