节点文献
不同预处理方式对萝卜小孢子活力的影响
Effect of Different Pretreatment on the Vigor of Microspores in Radish(Raphanus sativus L)
【摘要】 研究温度(低温和高温)及培养基(无碳源、甘露醇及秋水仙素)预处理对4个萝卜基因型小孢子活力的影响,得出的结果是:4℃低温条件下处理3 d的小孢子可保持较高的存活率,36℃高温处理后的小孢子存活率明显低于对照;加入蔗糖的培养基对提高小孢子存活率作用明显;甘露醇及秋水仙素处理也能提高萝卜小孢子存活率,其合适的浓度在不同萝卜基因型中存在差异。
【Abstract】 Effects of low temperature,high temperature,sugar-free,colchicines and mannitol pretreatment were studied on the survival rate of microspores in 4 radish cultivars.The result show that microspores had high survival rate under 4℃ after 3 days treatment,while 36℃ treatment reduced the survival rate.The media with sugar,mannitol and colchicines enhanced the vigor of microspores.And the concentrations of mannitol and colchicines had different effects on 4 radish cultivars.
【基金】 北京市科技新星项目(H020821470130);北京市自然科学基金项目(6062011)
- 【文献出处】 华北农学报 ,Acta Agriculturae Boreali-Sinica , 编辑部邮箱 ,2006年06期
- 【分类号】S631.1
- 【被引频次】18
- 【下载频次】260