【摘要】 为了建立针对山羊卵母细胞的理想体外成熟培养体系,对山羊卵泡卵母细胞的采集方法,FSH和LH对山羊卵泡卵母细胞体外成熟的作用,及培养时间对卵母细胞体外成熟的影响进行了研究。结果表明:采用切割法平均每枚卵巢采集到的A,B级卵母细胞的数量明显多于抽吸法;采用TCM199添加体积分数为10%FCS,1~2 mg/L 17β_E2,10 mmol/L Hepes,0.12 mg/mL丙酮酸钠,0.1 mg/mL链霉素和0.125 mg/mL青霉素,10 mg/L FSH及20~50 mg/L LH的培养体系,有利于山羊卵母细胞的体外成熟,并放出第一极体。山羊卵母细胞体外成熟培养20,22,24 h,成熟率显著高于培养18,26,30 h(P<0.05)。更多还原

【Abstract】 The experiments had been carried out to develop an improved in vitro maturation system for goat oocytes by comparing the collecting methods of oocytes and the effects of FSH,LH and culture time on goat oocytes in vitro maturation.The result had showed that a greater number of useable oocyte was obtained by slicing method than by aspiration method.An addition of 10% FCS,1-2 mg/L 17β-E-2,10 mmol/L Hepes,0.12 mg/mL pyruvate sodium,0.1 mg/mL streptomycin,0.125 mg/mL penicillin,10 mg/L FSH,20-50 mg/L LH in the TCM199 medium had been effective to goat oocyte maturation in vitro,and to the extruding of the first polar body.The rate of maturation of goat oocyte in vitro cultured for 20,22,24 hours had been remarkably higher than those cultured for 18,26,30 hours(P<0.05).更多还原