【摘要】 以192份玉米品种为材料,用7对SSR引物对毛细管电泳荧光检测和常规的变性PAGE银染检测两种SSR标记检测方法进行比较分析。结果表明,两种方法检测的SSR标记片段大小一致。毛细管电泳荧光检测法检测的结果更为精确、灵敏、高效,更适用于高通量材料的检测分析。对少量材料进行检测分析以及SSR标记的筛选时,使用常规的变性PAGE银染检测法更经济适用。更多还原

【Abstract】 Seven SSR primers and 192 maize hybrid were used to comparatively analyze SSR markers between capillary electrophoresis with fluorescence detection and denaturing PAGE(polyacrylamide gel electrophoresis) silver-staining detection.The results indicated that SSR markers fragment sizes explored by two decetion systems were relatively similar.Capillary electrophoresis with fluorescence detection has the advantages of high accuracy,sensitiveness,cost effectiveness and high-throught.Therefore,the capillary electrophoresis with fluorescence detection system is suggested to be suitable for scaning and analyzing large-scale material.While denaturing PAGE silver-staining detection would be used in examaining a few samples,especially in screening SSR markers.更多还原